These findings appear to be of considerable interest because, according to current views, key processes in lipid-mediated transfection such as lipoplex disassembly and DNA release within the cells are believed to take place upon cationic lipid mixing with cellular lipids. Indeed, X-ray structural studies show that the rate of DNA release from lipoplexes as well as transfection activity well correlate with non-lamellar phase progressions observed in cationic PC mixtures with membrane lipids. LIPOID E 20 O, Lecithin fraction with 40 phosphatidylcholine, organic certified (DEKO003), CAS-No. Lipid phase behavior is known to depend strongly on the chain molecular structure and the above relationships thus substantiate a view that cationic PC phase propensities are an important determinant of their activity. Maximum transfection was observed for ethyl phosphate PCs (EPCs) with monounsaturated 14:1 chains (total of 2 double bonds and 30 chain carbon atoms). A summary of studies on a set of ∼30 cationic PCs reveals the existence of a strong, systematic dependence of their transfection efficiency on the lipid hydrocarbon chain structure: transfection activity increases with increase of chain unsaturation from 0 to 2 double bonds per lipid and decreases with increase of chain length in the range ∼30–50 total number of chain carbon atoms. On this page, we’ll learn about the structures of these three types of lipids, as well as their functions in the. There are three main types of lipids: triglycerides, phospholipids, and sterols. Similarly to other cationic lipids, they form stable complexes (lipoplexes) with the polyanionic nucleic acids. Lipids are a family of organic compounds that are mostly insoluble in water, meaning they do not mix well with water. Examined here is a particularly attractive cationic lipid class, triester phosphatidylcholines (PCs) exhibiting low toxicities and good transfection efficiency. from publication: Phospholipids in Foods: Prooxidants or Antioxidants Lipid oxidation is one of the major causes of quality deterioration. Synthetic cationic lipids are presently the most widely used non-viral gene carriers. Download scientific diagram Phospholipid structures.
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